4UML
Crystal structure of ganglioside induced differentiation associated protein 2 (GDAP2) macro domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-04-12 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 70.840, 70.840, 97.549 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 57.320 - 1.900 |
| R-factor | 0.1978 |
| Rwork | 0.196 |
| R-free | 0.23089 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4iqy |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.465 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.570 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.100 | 1.350 |
| Number of reflections | 20255 | |
| <I/σ(I)> | 13.7 | 2.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.5 | 10.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 20% PEG3350, 10% ETHYLENE GLYCOL, 0.2 M SODIUM/POTASSIUM TARTRATE, pH 7.5 |
