4ULW
Crystal structure of the ROQ-domain of human ROQUIN1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 169.660, 29.785, 60.197 |
| Unit cell angles | 90.00, 100.94, 90.00 |
Refinement procedure
| Resolution | 44.390 - 1.910 |
| R-factor | 0.20201 |
| Rwork | 0.200 |
| R-free | 0.24144 |
| Structure solution method | OTHER |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.565 |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.390 | 1.980 |
| High resolution limit [Å] | 1.910 | 1.910 |
| Rmerge | 0.090 | 0.440 |
| Number of reflections | 23379 | |
| <I/σ(I)> | 9.08 | 2.01 |
| Completeness [%] | 99.2 | 95.7 |
| Redundancy | 3.2 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 20% (W/V) PEG 6000, 0.2 M LITHIUM CHLORIDE, 0.1 M TRIS/HCL PH 8.0 |
