4UHP
Crystal structure of the pyocin AP41 DNase-Immunity complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-07-16 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 |
| Unit cell lengths | 36.604, 75.627, 83.171 |
| Unit cell angles | 79.19, 78.05, 77.00 |
Refinement procedure
| Resolution | 40.236 - 2.000 |
| R-factor | 0.1882 |
| Rwork | 0.186 |
| R-free | 0.23470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bx1 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.667 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.240 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.180 | 0.940 |
| Number of reflections | 55279 | |
| <I/σ(I)> | 4.7 | 2.7 |
| Completeness [%] | 97.2 | 96.2 |
| Redundancy | 1.8 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 40% JEFFAMINE D2000, 0.1 M HEPES PH 6.5 |
