4UFK
Mouse Galactocerebrosidase complexed with dideoxy-imino-lyxitol DIL
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-02-28 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | H 3 2 |
Unit cell lengths | 248.820, 248.820, 77.850 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 73.218 - 2.402 |
R-factor | 0.1552 |
Rwork | 0.153 |
R-free | 0.19360 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3zr5 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.977 |
Data reduction software | xia2 |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 73.220 | 2.460 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.120 | 1.180 |
Number of reflections | 35733 | |
<I/σ(I)> | 15.1 | 2.1 |
Completeness [%] | 99.7 | 99.3 |
Redundancy | 10.4 | 10.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.8 | 0.2 M SODIUM ACETATE, 0.1 M SODIUM CACODYLATE (PH 6.8), 34% PEG 8000 |