4UF2
Deerpox virus DPV022 in complex with Bax BH3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-09 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 92.481, 92.481, 45.529 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.240 - 3.000 |
| R-factor | 0.2167 |
| Rwork | 0.214 |
| R-free | 0.27430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | DPV022_BIM |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.403 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 92.480 | 3.180 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.140 | 1.100 |
| Number of reflections | 4297 | |
| <I/σ(I)> | 10.2 | 1.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 6.9 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 20% PEG 6000, 0.2M MES PH 6.0, 0,2M AMMONIUM SULPHATE. |
