4UE8
Complex of D. melanogaster eIF4E with the 4E binding protein Thor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-05-16 |
Detector | DECTRIS PILATUS 6M |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 35.450, 73.290, 38.260 |
Unit cell angles | 90.00, 112.49, 90.00 |
Refinement procedure
Resolution | 36.645 - 1.100 |
R-factor | 0.1461 |
Rwork | 0.145 |
R-free | 0.16140 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4AXG CHAIN A |
RMSD bond length | 0.007 |
RMSD bond angle | 1.168 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE: 1.9_1692)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.600 | 1.130 |
High resolution limit [Å] | 1.100 | 1.100 |
Number of reflections | 70124 | |
<I/σ(I)> | 23.7 | 4 |
Completeness [%] | 95.8 | 88.7 |
Redundancy | 6 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.2 | 0.2M KNO3, 0.5% N-OCTYL-BETA-D-GLUCOSIDE, 18% PEG3350, pH 7.2 |