4UDR
Crystal structure of the H467A mutant of 5-hydroxymethylfurfural oxidase (HMFO)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-07-10 |
| Detector | DECTRIS PIXEL |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.820, 122.500, 73.270 |
| Unit cell angles | 90.00, 91.12, 90.00 |
Refinement procedure
| Resolution | 73.260 - 1.600 |
| R-factor | 0.17768 |
| Rwork | 0.176 |
| R-free | 0.21753 |
| Structure solution method | OTHER |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.915 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 21.000 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.130 | 0.570 |
| Number of reflections | 115432 | |
| <I/σ(I)> | 4.8 | 1.5 |
| Completeness [%] | 98.2 | 95.2 |
| Redundancy | 2.8 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 22% W/V PEG3350, 200 MM MAGNESIUM FORMATE |
