4UDQ
Crystal structure of 5-hydroxymethylfurfural oxidase (HMFO) in the reduced state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-03-19 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.550, 121.830, 72.490 |
| Unit cell angles | 90.00, 91.40, 90.00 |
Refinement procedure
| Resolution | 72.470 - 1.600 |
| R-factor | 0.1707 |
| Rwork | 0.168 |
| R-free | 0.21618 |
| Structure solution method | OTHER |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.899 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.000 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.120 | 0.740 |
| Number of reflections | 114344 | |
| <I/σ(I)> | 4.8 | 1.2 |
| Completeness [%] | 99.2 | 98.3 |
| Redundancy | 2.8 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 22% W/V PEG3350, 200 MM MAGNESIUM FORMATE |
