4UDM
Crystal structure of Im3 in complex with Y52A mutant of E3RNase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 92.854, 92.854, 76.942 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 38.470 - 2.960 |
| R-factor | 0.18569 |
| Rwork | 0.184 |
| R-free | 0.21378 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1e44 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.725 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.920 |
| High resolution limit [Å] | 2.970 | 2.970 |
| Rmerge | 0.080 | 0.070 |
| Number of reflections | 30491 | |
| <I/σ(I)> | 22 | 3.1 |
| Completeness [%] | 98.9 | 98.4 |
| Redundancy | 2.9 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 0.1M BIS-TRIS PH 6.5, 50MM CACL2,30%PEG550MME |
