4UDJ
Crystal structure of b-1,4-mannopyranosyl-chitobiose phosphorylase at 1.60 Angstrom in complex with beta-D-mannopyranose and inorganic phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-09-26 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 83.880, 140.840, 168.750 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 108.130 - 1.940 |
| R-factor | 0.15173 |
| Rwork | 0.150 |
| R-free | 0.19333 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4udi |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.853 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.000 | 2.040 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Rmerge | 0.160 | 0.590 |
| Number of reflections | 148390 | |
| <I/σ(I)> | 4 | 1.2 |
| Completeness [%] | 99.7 | 99.5 |
| Redundancy | 8.2 | 7.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | PROTEIN WAS CRYSTALLIZED FROM 17.5% PEG 3350, 200 MM NH4CL, pH 7.0 |
