4UD7
Structure of the stapled peptide YS-02 bound to MDM2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-07-03 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.148, 69.538, 78.464 |
| Unit cell angles | 90.00, 102.36, 90.00 |
Refinement procedure
| Resolution | 76.650 - 1.600 |
| R-factor | 0.1405 |
| Rwork | 0.138 |
| R-free | 0.17838 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ycr |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.478 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.000 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.060 | 0.690 |
| Number of reflections | 62522 | |
| <I/σ(I)> | 12.1 | 1.9 |
| Completeness [%] | 97.9 | 93.9 |
| Redundancy | 3.7 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4 | 0.1 M SODIUM CITRATE PH 4.0 AND 15 % PEG8000 |
