4UD5
Structural Plasticity of Cid1 Provides a Basis for its RNA Terminal Uridylyl Transferase Activity
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-01-25 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.800, 103.770, 76.390 |
| Unit cell angles | 90.00, 110.78, 90.00 |
Refinement procedure
| Resolution | 58.833 - 2.520 |
| R-factor | 0.1782 |
| Rwork | 0.176 |
| R-free | 0.21830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4e7x |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.881 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE: DEV_1772)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.830 | 2.600 |
| High resolution limit [Å] | 2.520 | 2.520 |
| Rmerge | 0.030 | 0.530 |
| Number of reflections | 30480 | |
| <I/σ(I)> | 23 | 2.1 |
| Completeness [%] | 98.4 | 98.7 |
| Redundancy | 3.4 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 20MM HEPES, 200MM NACL, 0.5MM TCEP, PH 7.0 |
