4UCI

X-ray structure and activities of an essential Mononegavirales L- protein domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-10-09
Detector	DECTRIS PILATUS
Spacegroup name	P 21 21 21
Unit cell lengths	80.220, 83.610, 182.620
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.200 - 2.210
R-factor	0.1909
Rwork	0.190
R-free	0.20090
Structure solution method	SAD
Starting model (for MR)	NONE
RMSD bond length	0.008
RMSD bond angle	0.970
Data reduction software	xia2
Data scaling software	xia2
Refinement software	BUSTER (2.10.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	83.600	9.880
High resolution limit [Å]	2.210	2.210
Rmerge	0.070
Number of reflections	62253
<I/σ(I)>	17.4
Completeness [%]	99.9
Redundancy	13.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1