4UCE
N-terminal globular domain of the RSV Nucleoprotein in complex with the Nucleoprotein Phosphoprotein interaction inhibitor M72
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-12-06 |
Detector | ADSC CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 34.050, 71.350, 178.400 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.950 |
R-factor | 0.2069 |
Rwork | 0.203 |
R-free | 0.24010 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2wj8 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.080 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | BUSTER (2.11.4) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 3.100 |
High resolution limit [Å] | 2.940 | 2.940 |
Rmerge | 0.170 | 0.480 |
Number of reflections | 8454 | |
<I/σ(I)> | 9 | 1.9 |
Completeness [%] | 86.8 | 61.8 |
Redundancy | 4.7 | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |