4UC7

N-terminal globular domain of the RSV Nucleoprotein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-09-22
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 21 21 21
Unit cell lengths	34.310, 72.130, 181.670
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.450
R-factor	0.2229
Rwork	0.218
R-free	0.26820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2wj8
RMSD bond length	0.010
RMSD bond angle	1.140
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	BUSTER (2.11.4)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.580
High resolution limit [Å]	2.450	2.450
Rmerge	0.080	0.350
Number of reflections	87163
<I/σ(I)>	13.3	1.5
Completeness [%]	88.3	47.7
Redundancy	5.7	1.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1