4UBQ
Crystal Structure of IMP-2 Metallo-beta-Lactamase from Acinetobacter spp.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-03-09 |
| Detector | RIGAKU RAXIS VII |
| Wavelength(s) | 1.540 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 37.942, 68.488, 88.322 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.160 - 2.300 |
| R-factor | 0.23548 |
| Rwork | 0.232 |
| R-free | 0.29864 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dd6 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.170 |
| Data scaling software | CrystalClear |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.200 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.255 | |
| Number of reflections | 10767 | |
| <I/σ(I)> | 6.6 | 2.3 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 6.81 | 6.62 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 0.1M citric acid/sodium citrate buffer, 0.2M sodium acetate, PEG 4000 |
