4UB1
DNA polymerase beta product complex with a templating adenine and 8-oxodGMP, 90 s
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-27 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.131, 80.800, 55.462 |
| Unit cell angles | 90.00, 109.58, 90.00 |
Refinement procedure
| Resolution | 31.962 - 2.341 |
| R-factor | 0.2278 |
| Rwork | 0.222 |
| R-free | 0.27720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3isb |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.061 |
| Refinement software | PHENIX ((phenix.refine: 1.7_650)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.390 |
| High resolution limit [Å] | 2.341 | 2.341 |
| Rmerge | 0.052 | 0.334 |
| Number of reflections | 34952 | |
| <I/σ(I)> | 24.3 | 2.3 |
| Completeness [%] | 97.3 | 77 |
| Redundancy | 3.5 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 291 | 50 mM imidazole, 350 mM sodium chloride, 17% PEG3350, pH 8.0 |
