4UAE

Importin alpha 3 delta IBB in complex with Influenza PB2 Nuclear Localization Domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X6A
Synchrotron site	NSLS
Beamline	X6A
Temperature [K]	100
Detector technology	CCD
Collection date	2013-10-26
Detector	ADSC QUANTUM 270
Wavelength(s)	1.006
Spacegroup name	P 41
Unit cell lengths	135.682, 135.682, 47.309
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	14.981 - 2.700
R-factor	0.1876
Rwork	0.185
R-free	0.23860
RMSD bond length	0.011
RMSD bond angle	1.073
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall
Low resolution limit [Å]	15.000
High resolution limit [Å]	2.700
Number of reflections	23809
<I/σ(I)>	11.1
Completeness [%]	98.8
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.1 M Na cacodylate, pH 6.5 0.1 M NaCl 1.1 M ammonium sulfate