4U9Z
Structure of the alpha-tubulin acetyltransferase alpha-TAT1/Mec-17 in complex with CoA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2012-06-23 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.645, 34.329, 64.226 |
| Unit cell angles | 90.00, 103.03, 90.00 |
Refinement procedure
| Resolution | 31.976 - 1.802 |
| R-factor | 0.2181 |
| Rwork | 0.216 |
| R-free | 0.25780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3vwd |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.331 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER (2.4.0) |
| Refinement software | PHENIX (phenix.refine: 1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 4.880 | 1.800 |
| Rmerge | 0.084 | 0.057 | 0.367 |
| Total number of observations | 63548 | ||
| Number of reflections | 17372 | ||
| <I/σ(I)> | 11.2 | ||
| Completeness [%] | 99.8 | 97.2 | 100 |
| Redundancy | 3.7 | 3.4 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 0.1M Tris, 25% PEG 3350 |
