4U9D
Crystal Structure of the Zn-directed tetramer of the engineered cyt cb562 variant, AB3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-01-20 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 61 |
| Unit cell lengths | 54.830, 54.830, 237.820 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.480 - 2.500 |
| R-factor | 0.2409 |
| Rwork | 0.238 |
| R-free | 0.29580 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.935 |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 47.480 |
| High resolution limit [Å] | 2.500 |
| Number of reflections | 13889 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 18% PEG 400 in 20 mM Tris (pH 8.5) and 0.2 M MgCl2 |
