4U8T
Crystal structure of YTH domain of Zygosaccharomyces rouxii MRB1 protein in complex with N6-Methyladenosine RNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-04-01 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9790 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 117.390, 117.390, 380.928 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 24.921 - 2.700 |
R-factor | 0.2009 |
Rwork | 0.199 |
R-free | 0.24520 |
Structure solution method | SAD |
RMSD bond length | 0.015 |
RMSD bond angle | 1.336 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHARP |
Refinement software | PHENIX ((phenix.refine: 1.9_1675)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 25.000 | 25.000 | 2.800 |
High resolution limit [Å] | 2.700 | 5.800 | 2.700 |
Rmerge | 0.137 | 0.105 | 0.498 |
Total number of observations | 186118 | ||
Number of reflections | 41339 | ||
<I/σ(I)> | 6.6 | ||
Completeness [%] | 94.2 | 89.8 | 96.1 |
Redundancy | 4.5 | 4.4 | 4.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICROBATCH | 277 | 0.8 M ammonium dihydrogen phosphate |