4U88

Structure of the DNA-Binding Domain of the Response Regulator SaeR from Staphylococcus aureus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13C1
Synchrotron site	NSRRC
Beamline	BL13C1
Temperature [K]	173
Detector technology	CCD
Collection date	2014-05-10
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	31.966, 61.024, 116.759
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.521 - 2.355
R-factor	0.201
Rwork	0.194
R-free	0.26430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ixa
RMSD bond length	0.009
RMSD bond angle	1.163
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.350
Number of reflections	9992
<I/σ(I)>	19.7
Completeness [%]	99.6
Redundancy	9.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	5% v/v Tacsimate, pH 7.0, 0.1M HEPES pH 7.0, 10% w/v Polyethylene glycol monomethyl ether 5000