4U7Y
Structure of the complex of VPS4B MIT and IST1 MIM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-02-19 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97872 |
| Spacegroup name | P 6 2 2 |
| Unit cell lengths | 78.401, 78.401, 67.642 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.820 - 2.502 |
| R-factor | 0.2276 |
| Rwork | 0.225 |
| R-free | 0.27130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1wr0 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.997 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: dev_1593)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.820 | |
| High resolution limit [Å] | 2.500 | |
| Rmerge | 0.112 | 0.387 |
| Number of reflections | 4455 | |
| <I/σ(I)> | 21.1 | 6.4 |
| Completeness [%] | 96.9 | 98.2 |
| Redundancy | 12.3 | 12.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.3 | 299 | 2.58 M Na-malonate |
