4U7S
Homodimeric Single Domain Antibody (sdAb) against Staphylococcal enterotoxin B (SEB) Crystallized for 5 months
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR-H |
| Temperature [K] | 150 |
| Detector technology | CCD |
| Collection date | 2012-04-25 |
| Detector | BRUKER SMART 6000 |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 67.776, 72.978, 117.155 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.660 - 2.070 |
| R-factor | 0.23267 |
| Rwork | 0.232 |
| R-free | 0.24207 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4tyu |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.079 |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.660 | 2.160 |
| High resolution limit [Å] | 2.070 | 2.070 |
| Rmerge | 0.066 | 0.196 |
| Number of reflections | 17180 | |
| <I/σ(I)> | 24.28 | 5.88 |
| Completeness [%] | 94.9 | 79.1 |
| Redundancy | 7.79 | 2.45 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.1 | 290 | 0.1 M Citric acid, pH 4.1, 20% Glycerol, 1.6M Ammonium sulfate |
