4U7B
Crystal structure of a pre-cleavage Mos1 transpososome
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 77 |
| Detector technology | PIXEL |
| Collection date | 2013-05-04 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 96.471, 340.090, 160.360 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.750 - 3.090 |
| R-factor | 0.2301 |
| Rwork | 0.228 |
| R-free | 0.26140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hos |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.969 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 92.500 | 3.270 |
| High resolution limit [Å] | 3.090 | 3.090 |
| Rmerge | 0.134 | 0.442 |
| Number of reflections | 69135 | |
| <I/σ(I)> | 8.9 | 3.4 |
| Completeness [%] | 99.7 | 99.7 |
| Redundancy | 8 | 8.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 290 | 100 mM Ammonium acetate, 20 mM MgCl2, 50 mM Hepes pH 7.0, 5 % (w/v) PEG 8,000 |
