4U78
Octameric RNA duplex soaked in copper(II)chloride
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-10-27 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.37 |
| Spacegroup name | H 3 |
| Unit cell lengths | 46.810, 46.810, 53.380 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.280 - 1.501 |
| R-factor | 0.1773 |
| Rwork | 0.174 |
| R-free | 0.20610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4u3l |
| RMSD bond length | 0.036 |
| RMSD bond angle | 0.403 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.280 | 1.553 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.032 | 0.350 |
| Number of reflections | 6864 | |
| <I/σ(I)> | 46.45 | 4.5 |
| Completeness [%] | 98.4 | 90.32 |
| Redundancy | 12.6 | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293.15 | 28% PEG 400, 0.2 M calcium chloride, 0.002 M spermine, 0.05 M hepes sodium buffer, pH 7.5 |
