4U68

Crystal structure of Rhino chromodomain in complex with H3K9me3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2013-11-15
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9791
Spacegroup name	C 1 2 1
Unit cell lengths	50.946, 67.679, 79.750
Unit cell angles	90.00, 106.46, 90.00

Refinement procedure

Resolution	25.494 - 1.800
R-factor	0.1862
Rwork	0.184
R-free	0.20620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3lwe
RMSD bond length	0.008
RMSD bond angle	1.155
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.500	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.067	0.410
Number of reflections	23980
<I/σ(I)>	18.4	3.5
Completeness [%]	99.4	99.5
Redundancy	3.4	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	290	30 %(v/v) 2-methyl-2,4-pentanediol, 0.1 M sodium acetate, 25 % (w/v) PEG 1500