4U63
Crystal structure of a bacterial class III photolyase from Agrobacterium tumefaciens at 1.67A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-03 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.918420 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 81.867, 81.867, 195.952 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.300 - 1.670 |
| R-factor | 0.15736 |
| Rwork | 0.156 |
| R-free | 0.18047 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1u3c |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.229 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SHELXDE |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.300 | 1.760 |
| High resolution limit [Å] | 1.670 | 1.670 |
| Rmerge | 0.085 | 0.930 |
| Number of reflections | 89158 | |
| <I/σ(I)> | 13.7 | 2.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.2 | 9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.8 | 282 | 3 M ammonium sulfate, 10% (v/v) glycerol, 75 mM sodium chloride, 12.5 mM tris (hydroxymethyl) amino methane, 1.25 mM ethylenediaminetetraacetic acid. |
