4U5T
Crystal Structure of VBP Leucine Zipper with Bound Arylstibonic Acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-06-19 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 61 |
| Unit cell lengths | 68.840, 68.840, 77.230 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.420 - 3.301 |
| R-factor | 0.2617 |
| Rwork | 0.260 |
| R-free | 0.27810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.622 |
| Data reduction software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 34.420 | 3.400 | |
| High resolution limit [Å] | 3.300 | 20.000 | 3.300 |
| Rmerge | 0.115 | 0.013 | 0.495 |
| Rmeas | 0.133 | 0.015 | 0.573 |
| Total number of observations | 12301 | ||
| Number of reflections | 3054 | 13 | 238 |
| <I/σ(I)> | 14.86 | 75.2 | 3.51 |
| Completeness [%] | 96.3 | 86.7 | 92.6 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 295 | 0.2M ammonium phos., 20% PEG3350, 3% trimethylamine N-oxide |
