4U5D
Crystal structure of GluA2, con-ikot-ikot snail toxin, partial agonist KA and postitive modulator (R,R)-2b complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-08-02 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 161.510, 367.580, 109.380 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.995 - 3.576 |
R-factor | 0.2579 |
Rwork | 0.256 |
R-free | 0.29720 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.753 |
Data scaling software | XDS |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 97.620 |
High resolution limit [Å] | 3.520 |
Number of reflections | 77844 |
<I/σ(I)> | 5.1 |
Completeness [%] | 96.0 |
Redundancy | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.1 M MES pH 5.8-6.3, 0.1 M NaCl, 5%-6% PEG3350 |