4U54

IMPORTIN-ALPHA MINOR NLS SITE INHIBITOR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-11-05
Detector	PSI PILATUS 6M
Wavelength(s)	0.9686
Spacegroup name	P 21 21 21
Unit cell lengths	78.424, 91.298, 98.816
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.033 - 2.410
R-factor	0.1879
Rwork	0.186
R-free	0.22740
Data reduction software	XSCALE
Refinement software	PHENIX ((phenix.refine: dev_1702))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.500
High resolution limit [Å]	2.410	2.410
Number of reflections	27498
<I/σ(I)>	11	2.3
Completeness [%]	98.4	99.4
Redundancy	4.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	291	See publication for details. Precipitant was 1.05-1.2 M ammonium sulphate, 0.1M citrate pH 6.0, 20-40 mM DTT.