4U4W

Structure of a nitrate/nitrite antiporter NarK in nitrate-bound occluded state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	CCD
Collection date	2013-06-28
Detector	RAYONIX MX225HE
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	68.780, 86.100, 221.550
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.350 - 2.400
R-factor	0.2198
Rwork	0.219
R-free	0.23680
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.671
Refinement software	PHENIX ((phenix.refine: 1.8.3_1479))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.400
Number of reflections	50895
<I/σ(I)>	7
Completeness [%]	97.2
Redundancy	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	5.8	293	31% PEG 500DME, 100 mM ammonium nitrate, 100 mM MES-NaOH