4U4T

Structure of a nitrate/nitrite antiporter NarK in nitrate-bound inward-open state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	CCD
Collection date	2011-12-18
Detector	RAYONIX MX-225
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	35.394, 109.358, 126.040
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.300 - 2.400
R-factor	0.2185
Rwork	0.217
R-free	0.24750
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.169
Refinement software	PHENIX ((phenix.refine: 1.8.3_1479))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.400
Number of reflections	19921
<I/σ(I)>	6.79
Completeness [%]	99.8
Redundancy	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	6	293	32-36% PEG 400, 10 mM zinc acetate, 3% D-sucrose, 100 mM MES-NaOH