4U4H
Crystal Structure of HSV-1 UL21 N-terminal Domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-05-01 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 108.346, 108.346, 65.236 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.809 - 2.050 |
R-factor | 0.1683 |
Rwork | 0.166 |
R-free | 0.22140 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.075 |
Data reduction software | HKL-2000 |
Phasing software | SHARP |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.700 | 2.090 |
High resolution limit [Å] | 2.050 | 2.050 |
Rmerge | 0.061 | |
Number of reflections | 14650 | |
<I/σ(I)> | 23.3 | 2.5 |
Completeness [%] | 99.8 | 99.6 |
Redundancy | 5.3 | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | Ammonium Sulfate, HEPES, 2-methyl-2,4-pentanediol |