4U4G
Structure of GluA2* in complex with competitive antagonist ZK 200775
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-12-17 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 91.999, 310.360, 109.502 |
| Unit cell angles | 90.00, 94.82, 90.00 |
Refinement procedure
| Resolution | 50.000 - 4.490 |
| R-factor | 0.218 |
| Rwork | 0.217 |
| R-free | 0.24800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3h5v 3kg2 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.673 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (PHENIX.REFINE: 1.8_1069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 4.650 |
| High resolution limit [Å] | 4.490 | 4.490 |
| Rmerge | 0.098 | 0.997 |
| Number of reflections | 36039 | |
| <I/σ(I)> | 12.7 | 1.48 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 277 | 7-11% PEG 20,000, 0.1 M MES |
