4U4G

Structure of GluA2* in complex with competitive antagonist ZK 200775

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2012-12-17
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9792
Spacegroup name	P 1 21 1
Unit cell lengths	91.999, 310.360, 109.502
Unit cell angles	90.00, 94.82, 90.00

Refinement procedure

Resolution	50.000 - 4.490
R-factor	0.218
Rwork	0.217
R-free	0.24800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3h5v 3kg2
RMSD bond length	0.003
RMSD bond angle	0.673
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (PHENIX.REFINE: 1.8_1069)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	4.650
High resolution limit [Å]	4.490	4.490
Rmerge	0.098	0.997
Number of reflections	36039
<I/σ(I)>	12.7	1.48
Completeness [%]	99.8	100
Redundancy	3.8	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE		277	7-11% PEG 20,000, 0.1 M MES