4U4A

Complex Structure of BRCA1 BRCT with singly phospho Abraxas

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	2011-02-03
Detector	MARRESEARCH
Wavelength(s)	0.900
Spacegroup name	P 41 21 2
Unit cell lengths	187.500, 187.500, 85.350
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	59.890 - 3.510
R-factor	0.3042
Rwork	0.301
R-free	0.36180
RMSD bond length	0.010
RMSD bond angle	1.469
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall
Low resolution limit [Å]	59.890
High resolution limit [Å]	3.510
Number of reflections	15491
<I/σ(I)>	9.5
Completeness [%]	100.0
Redundancy	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	295	0.01M Cobalt Chloride, 0.1 M(MES), pH 6.5, 30% PEG Mono Methyl Ether (MME) 5000