4U3R

Octameric RNA duplex co-crystallized with cobalt(II)chloride

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-10-27
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.61
Spacegroup name	H 3
Unit cell lengths	46.977, 46.977, 53.300
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.339 - 1.701
R-factor	0.168
Rwork	0.165
R-free	0.19760
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4u3l
RMSD bond length	0.019
RMSD bond angle	0.401
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.340	1.760
High resolution limit [Å]	1.701	1.701
Rmerge	0.037	0.091
Number of reflections	4678
<I/σ(I)>	55.81	12.1
Completeness [%]	96.8	84.68
Redundancy	15.7	4.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293.15	25% MPD, 0.02 M magnesium sulfate, 0.0005 M spermine, 0.05 M potassium cacodylate pH 6.0