4U2Q

Full-length AMPA subtype ionotropic glutamate receptor GluA2 in complex with partial agonist kainate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2013-01-12
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	104.940, 148.650, 337.060
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	83.084 - 3.525
R-factor	0.2709
Rwork	0.269
R-free	0.31750
RMSD bond length	0.004
RMSD bond angle	0.881
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall
Low resolution limit [Å]	83.084
High resolution limit [Å]	3.500
Number of reflections	39983
<I/σ(I)>	6.45
Completeness [%]	80.8
Redundancy	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277.15	0.1 M MES, 4.8-5.3% PEG6000, 3.5% TMAO, 6 mM taurine