4U24

Crystal structure of the E. coli ribosome bound to dalfopristin.

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-04-04
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.1
Spacegroup name	P 21 21 21
Unit cell lengths	210.210, 433.030, 619.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.968 - 2.900
R-factor	0.22
Rwork	0.220
R-free	0.26390
RMSD bond length	0.006
RMSD bond angle	1.069
Data reduction software	XDS
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	70.000		3.000
High resolution limit [Å]	2.900	50.000	2.900
Rmerge	0.124		0.769
Rmeas	0.144		0.935
Total number of observations	3534355
Number of reflections	1169643		103248
<I/σ(I)>	7.04		0.63
Completeness [%]	94.9		87.1
Redundancy	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	6.5	291	PEG8k, MPD
1	MICROBATCH	6.5	291	PEG8k, MPD