4U23

GluA2flip sLBD complexed with FW and (R,R)-2b crystal form F

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2013-01-02
Detector	ADSC QUANTUM 315r
Wavelength(s)	1
Spacegroup name	C 2 2 21
Unit cell lengths	60.090, 91.290, 111.940
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.266 - 1.673
R-factor	0.2198
Rwork	0.218
R-free	0.24990
RMSD bond length	0.006
RMSD bond angle	1.060
Data reduction software	XDS
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall
Low resolution limit [Å]	42.270
High resolution limit [Å]	1.670
Number of reflections	35750
<I/σ(I)>	22
Completeness [%]	99.6
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M bicine/Trizma base, pH 8.5, 0.09 M Morpheus nitrate-phosphate-sulfate, 10% w/v PEG8K, 20% (v/v) ethylene glycol