4U21
GluA2flip sLBD complexed with FW and (R,R)-2b crystal form E
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-01-02 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 97.290, 121.320, 47.740 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.150 - 1.391 |
R-factor | 0.1834 |
Rwork | 0.183 |
R-free | 0.19820 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.148 |
Data reduction software | XDS |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 45.150 |
High resolution limit [Å] | 1.390 |
Number of reflections | 110875 |
<I/σ(I)> | 18.2 |
Completeness [%] | 97.2 |
Redundancy | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | phosphate-citrate, pH 4.2, 0.2 M Li sulfate, 20% PEG1K |