4U1Q
Crystal structure of S-adenosylmethionine-dependent methyltransferase SibL in complex with 3HK and SAH
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13B1 |
Synchrotron site | NSRRC |
Beamline | BL13B1 |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2013-09-12 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9789 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 93.304, 112.783, 143.139 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.529 - 2.085 |
R-factor | 0.1722 |
Rwork | 0.171 |
R-free | 0.21320 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.086 |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.180 |
High resolution limit [Å] | 2.085 | 2.085 |
Number of reflections | 89602 | |
<I/σ(I)> | 23.2 | 4.2 |
Completeness [%] | 99.8 | 100 |
Redundancy | 7.3 | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 2.0 M Ammonium sulfate 0.1 M TRIS hydrochloride pH 8.5 |