4U0C
Hexameric HIV-1 CA in complex with Nup153 peptide, P6 crystal form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04-1 |
Synchrotron site | Diamond |
Beamline | I04-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-01-20 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.92 |
Spacegroup name | P 6 |
Unit cell lengths | 91.469, 91.469, 57.001 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 35.670 - 1.770 |
R-factor | 0.1923 |
Rwork | 0.191 |
R-free | 0.21080 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3h47 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.878 |
Data reduction software | MOSFLM |
Data scaling software | Aimless (0.2.18) |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 35.670 | 35.670 | 1.810 |
High resolution limit [Å] | 1.770 | 9.050 | 1.770 |
Rmerge | 0.109 | 0.033 | 0.942 |
Rpim | 0.066 | 0.019 | 0.581 |
Total number of observations | 79253 | 745 | 4573 |
Number of reflections | 24947 | ||
<I/σ(I)> | 7.3 | 21.4 | 1.6 |
Completeness [%] | 95.1 | 89.1 | 95.8 |
Redundancy | 3.2 | 4 | 3.2 |
CC(1/2) | 0.996 | 0.999 | 0.392 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9.5 | 290 | 30% v/v PEG 400, 0.1 M CHES |