4TZU

Crystal Structure of Murine Cereblon in Complex with Pomalidomide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2012-08-16
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.00
Spacegroup name	I 2 3
Unit cell lengths	143.340, 143.340, 143.340
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.000
R-factor	0.1979
Rwork	0.196
R-free	0.23920
RMSD bond length	0.017
RMSD bond angle	1.710
Data scaling software	SCALA (3.3.20)
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	101.357	41.379	2.110
High resolution limit [Å]	2.000	6.320	2.000
Rmerge		0.096	0.204
Rmeas	0.090
Rpim	0.050	0.066	0.134
Total number of observations	88108	2865	12968
Number of reflections	30305
<I/σ(I)>	10.1	14.3	5.4
Completeness [%]	92.3	86.7	92.4
Redundancy	2.9	3.1	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	293	100mM Sodium Acetate pH5, 600-800mM Ammonium Sulfate.