4TZO

Structure of C. elegans HTP-1 bound to HIM-3 closure motif

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2013-12-05
Detector	ADSC QUANTUM 315
Wavelength(s)	.979
Spacegroup name	P 1 21 1
Unit cell lengths	66.897, 107.873, 82.968
Unit cell angles	90.00, 90.10, 90.00

Refinement procedure

Resolution	45.221 - 2.400
R-factor	0.2475
Rwork	0.246
R-free	0.28220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4tzl
RMSD bond length	0.004
RMSD bond angle	0.770
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.400
Number of reflections	43476
<I/σ(I)>	6.3
Completeness [%]	94.1
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298.15	15-22% PEG 3350