4TZA

TGP, an extremely thermostable green fluorescent protein created by structure-guided surface engineering

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2011-09-15
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.542
Spacegroup name	P 21 21 21
Unit cell lengths	94.200, 141.220, 69.090
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.586 - 1.900
R-factor	0.174538516473
Rwork	0.174
R-free	0.20031
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.234
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHENIX ((phenix.refine: 1.9_1685))
Refinement software	PHENIX ((phenix.refine: 1.9_1685))

Data quality characteristics

	Overall
Low resolution limit [Å]	28.600
High resolution limit [Å]	1.900
Rmerge	0.062
Number of reflections	72746
<I/σ(I)>	12.3
Completeness [%]	99.2
Redundancy	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	20% w/v PEG MME 2000, 0.1 M Tris-HCl pH 7.0