4TZ7
Crystal structure of type I phosphatidylinositol 4-phosphate 5-kinase alpha from Zebrafish
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2012-02-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.075 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 88.946, 88.946, 157.074 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.309 - 3.310 |
| R-factor | 0.21261 |
| Rwork | 0.207 |
| R-free | 0.26766 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.404 |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 40.000 |
| High resolution limit [Å] | 3.300 |
| Number of reflections | 8877 |
| <I/σ(I)> | 30.1 |
| Completeness [%] | 99.5 |
| Redundancy | 13.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | PEG 20,000 |
