4TZ0

DEAD-box helicase Mss116 bound to ssRNA and GDP-BeF

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.3
Synchrotron site	ALS
Beamline	5.0.3
Temperature [K]	100
Detector technology	CCD
Collection date	2013-01-28
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0000
Spacegroup name	P 21 21 2
Unit cell lengths	89.979, 126.614, 55.553
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.270 - 2.350
R-factor	0.2324
Rwork	0.231
R-free	0.26000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.710
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall
Low resolution limit [Å]	47.270
High resolution limit [Å]	2.350
Rmerge	0.133
Number of reflections	27147
<I/σ(I)>	7
Completeness [%]	99.9
Redundancy	19.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	295	4 % tacsimate, pH 8.0, 12 % PEG 3350