4TYU
Homodimeric Single Domain Antibody (sdAb) against Staphylococcal enterotoxin B (SEB)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR-H |
| Temperature [K] | 150 |
| Detector technology | CCD |
| Collection date | 2012-01-12 |
| Detector | Bruker Platinum 135 |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 67.300, 72.240, 116.050 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.330 - 2.130 |
| R-factor | 0.2402 |
| Rwork | 0.239 |
| R-free | 0.26316 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4dka |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.035 |
| Data scaling software | PROTEUM PLUS |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.130 | 2.220 |
| High resolution limit [Å] | 2.120 | 2.120 |
| Rmerge | 0.139 | 0.365 |
| Number of reflections | 15098 | |
| <I/σ(I)> | 12.74 | 4.88 |
| Completeness [%] | 92.6 | 60.9 |
| Redundancy | 6.45 | 2.49 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.1 | 290 | 0.1 M citric acid pH 4.1, 1.6 M ammonium sulfate, 20 % glycerol |
