4TYP
Crystal structure of an adenylate kinase mutant--AKm1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-04-20 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.97934 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.641, 123.338, 86.693 |
| Unit cell angles | 90.00, 98.28, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.900 |
| R-factor | 0.28977 |
| Rwork | 0.288 |
| R-free | 0.32879 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4mkg |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.303 |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.164 | 0.611 |
| Number of reflections | 18989 | |
| <I/σ(I)> | 5.9 | 2.3 |
| Completeness [%] | 94.0 | 94.1 |
| Redundancy | 3.5 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 18% (w/v) polyethylene glycol 3350, 100mM lithium sulfate, 100mM Bis-Tris pH 5.5 |
